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    Biolegend LEAF™ Purified anti-human CD3 Antibody

    • 更新時間:  2023-07-25
    • 產(chǎn)品型號:  317315
    • 簡單描述
    • Clone:OKT3 (See other available formats)
      Isotype: Mouse IgG2a, κ
      Isotype Control:LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl
    詳細(xì)介紹

    LEAF™ Purified anti-human CD3 Antibody

    Product Details
     
     
     All CD3 Reagents
    Clone:OKT3 (See other available formats)
    Isotype:Mouse IgG2a, κ
    Isotype Control:LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl
    Reactivity:Human
    Formulation:0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
    Preparation:The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
    Concentration:1.0 mg/ml
    Storage & Handling:The CD3 antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
    Application:

    FC - Quality tested
    IHC, Activ - Reported in the literature

    Recommended Usage:

    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

    COA:

    Enter Lot#:     

    Application
    Notes:

    Clone OKT3 can block the binding of clones SK7 and UCHT1.8 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

    Application
    References:

    1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
    2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
    3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
    4. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
    5. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
    6. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
    7. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
    8. Li B, et al. 2005. Immunology 116:487.

     Publication Library
    LEAF™ <!Low Endotoxin, Azide-Free>Purified anti-human CD3 Antibody OKT3 Image 1

    Human peripheral blood lymphocytes stained with LEAF™ purified OKT3, followed by anti-mouse IgG FITC



    Compare all formats 
     
     
     
    Description:

    CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

    Other Names:T3, CD3ε
    Structure:Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
    Distribution:Mature T and NK-T cells, thymocyte differentiation
    Function:Antigen recognition, signal transduction, T cell activation
    Ligand Receptor:Peptide antigen bound to MHC
    Antigen
    References:

    1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 
    2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 
    3. Lanier L, et al. 1986. J. Immunol. 137:2501.

    GeneID:916 
    Latest Publications:View the latest CD3 articles on HighwirePress.com
    UniProt:View information about CD3 on UniProt.org
    Keywords:LEAF™ Purified anti-human CD3, OKT3, LEAF™, T3, CD3ε
    Related Products
     
    DescriptionCloneApplications
    LEAF™ Purified Mouse IgG2a, κ Isotype CtrlMOPC-173FC, WB, IP, ICFC, ICC, IF, IHC, FA
    Cell Staining Buffer FC, ICC, ICFC
    RBC Lysis Buffer (10X) FC, ICFC


    Other Formats
     
    DescriptionCloneApplications
    Alexa Fluor® 488 anti-human CD3OKT3FC
    Alexa Fluor® 647 anti-human CD3OKT3FC
    APC anti-human CD3OKT3FC
    Biotin anti-human CD3OKT3FC
    Brilliant Violet 510™ anti-human CD3OKT3FC
    Brilliant Violet 605™ anti-human CD3OKT3FC
    Brilliant Violet 650™ anti-human CD3OKT3FC
    Brilliant Violet 711™ anti-human CD3OKT3FC
    Brilliant Violet 785™ anti-human CD3OKT3FC
    FITC anti-human CD3OKT3FC
    Pacific Blue™ anti-human CD3OKT3FC
    PE anti-human CD3OKT3FC
    PE/Cy7 anti-human CD3OKT3FC
    PerCP/Cy5.5 anti-human CD3OKT3FC
    Purified anti-human CD3OKT3FC, IHC
    Ultra-LEAF™ Purified anti-human CD3OKT3FC, IHC, Activ

     

     

     

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