• <code id="i6oi8"><delect id="i6oi8"></delect></code>
    <li id="i6oi8"><input id="i6oi8"></input></li>
    <rt id="i6oi8"><acronym id="i6oi8"></acronym></rt>
  • <rt id="i6oi8"><acronym id="i6oi8"></acronym></rt>
    您好,歡迎進入上海力敏實業有限公司網站!
    一鍵分享網站到:
    您現在的位置:首頁 >> 產品中心 >> 生化試劑、抗體、血清 >> 抗體/蛋白 >> 05-321millipore Phosphotyrosine Antibody, clone 4G10抗體

    millipore Phosphotyrosine Antibody, clone 4G10抗體

    • 更新時間:  2023-07-25
    • 產品型號:  05-321
    • 簡單描述
    • millipore Phosphotyrosine Antibody, clone 4G10抗體
      規格:100ug
      現貨*!
    詳細介紹

    millipore Phosphotyrosine Antibody, clone 4G10抗體

    05-321 | Anti-Phosphotyrosine Antibody, clone 4G10

    Replacement Information    05-321 is a recommended replacement for MAB3090

    millipore Phosphotyrosine Antibody, clone 4G10抗體

    重要規格表

    Species ReactivityKey ApplicationsHostFormatAntibody Type
    AICC, WB, IP, IHCMPurifiedMonoclonal Antibody

    錨點錨點錨點錨點錨點

    Description 錨點
    Catalogue Number05-321
    ReplacesMAB3090
    Brand FamilyUpstate 
    錨點
    Trade Name
    • Upstate
    DescriptionAnti-Phosphotyrosine Antibody, clone 4G10?
    Background InformationSome of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. 
    The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

    錨點錨點錨點

    Product Information 錨點
    FormatPurified
    Control
    • Untreated A-431 (negative control) and EGF treated A-431 (positive control) whole cell lysates.
      Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediay, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
    Presentation0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4. Liquid at 2-8°C.
    IgG2bκ mouse monocolonal antibody produced in vitro by mouse-mouse hybridoma 4G10? (FOX-NY [NS-1 derivative] myeloma x spleen cells).

    錨點錨點錨點

    Applications 錨點
    ApplicationAnti-Phosphotyrosine Antibody, clone 4G10 detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is validated for use in IC, IH, IP, WB and is backed by hundreds of publications
    Key Applications
    • Immunocytochemistry
    • Western Blotting
    • Immunoprecipitation
    • Immunohistochemistry
    Application NotesImmunoprecipitation: 
    2-4 μg of a previous lot immunoprecipitated quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10 cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add: 0.2 mM sodium orthovanadate to the lysis buffer.

    錨點錨點錨點錨點錨點錨點錨點錨點錨點錨點錨點

    Biological Information 錨點
    ImmunogenPhosphotyramine-KLH
    Clone4G10?
    ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
    HostMouse
    SpecificityThis antibody recognizes tyrosine-phosphorylated proteins from all species.
    IsotypeIgG2bκ
    Species Reactivity
    • All
    Antibody TypeMonoclonal Antibody
    Modifications
    • Phosphorylation
    Purification MethodProtein G-Sepharose? Chromatography
    Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

    錨點錨點

    Product Usage Statements 錨點
    Quality AssuranceRoutinely evaluated on EGF-treated human A431 carcinoma cells.

    Western Blot Analysis: 
    0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; , Druker, B. J., 1989; Kanakura, Y., 1991).
    Usage Statement
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

    錨點

    Storage and Shipping Information 錨點
    Storage ConditionsStable for 1 year at 2-8°C from date of receipt. 

    NOTE: DO NOT FREEZE. 
    For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8oC. Save the supernatant for application.

    錨點

    Packaging Information 錨點
    Material Size100 μg


    現貨*,咨詢和選購!


    留言框

    • 產品:

    • 您的單位:

    • 您的姓名:

    • 聯系電話:

    • 常用郵箱:

    • 省份:

    • 詳細地址:

    • 補充說明:

    • 驗證碼:

      請輸入計算結果(填寫阿拉伯數字),如:三加四=7
    友情鏈接: 等離子去膠機       電液伺服萬能材料試驗機       電液伺服液壓萬能試驗機       電子拉伸萬能試驗機       材料拉伸試驗機       金剛石鉆孔機       北斗定位器       控溫式遠紅外消煮爐       切膠儀       無塵紙       金屬液氮冷凍箱       傳力接頭       磁座鉆       LEM電壓傳感器       鋁儲罐      
    亚洲а∨天堂久久精品9966| 亚洲人成无码网站久久99热国产| 伊人久久影院大香线蕉| 97超级碰碰碰久久久久| 久久精品一区二区三区四区| 亚洲国产成人乱码精品女人久久久不卡| 久久精品人人做人人妻人人玩| 伊人久久大香线蕉综合影| 久久精品国产亚洲AV电影网| 久久香蕉超碰97国产精品| 狠狠色婷婷久久综合频道日韩| 精品久久精品久久| 91精品久久久久久无码| 久久成人免费大片| 7777久久久国产精品消防器材| 国产精品久久久久久久伊一| 97热久久免费频精品99| 久久免费的精品国产V∧| 久久国产精品99精品国产987| 国产精品成人久久久久三级午夜电影| 伊人久久五月丁香综合中文亚洲| 久久人人爽人人爽人人AV | 狠狠色丁香婷婷久久综合不卡| 国产精品无码久久av| 欧美成人秋霞久久AA片| 亚洲美女aⅴ久久久91| 日韩乱码人妻无码中文字幕久久| 久久这里只有精品首页| 中文字幕乱码久久午夜| 久久精品亚洲乱码伦伦中文| 色综合久久88色综合天天 | 国产精品久久久久久久小说| 国产精品久久久久蜜芽| 国产色丁香久久综合| 久久综合丝袜长腿丝袜| 精品熟女少妇a∨免费久久| 久久免费看少妇高潮V片特黄| 日本一区精品久久久久影院| 久久国产精品久久| 久久精品国产精品国产精品污| 亚洲乱码精品久久久久..|